Anti-PAX3; clone 7D8G7

Application

ELISA: A representative lot was used on control antigen (100 ng) and Antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).

Detect PAX3 using this Anti-PAX3, clone 7D8G7 Antibody validated for use in western blotting & ELISA.

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Paired box protein Pax-3, also known as HuP2, and encoded by the gene name PAX3 or HUP2, is a transcription factor that may be involved in regulating cell proliferation, migration, and apoptosis. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma.

Immunogen

Purified recombinant fragment of human PAX3 expressed in E. Coli

Physical form

Mouse monoclonal IgG1 ascitic fluid with up to 0.1% sodium azide.

Unpurified

Quality

Evaluated by Western Blotting in A549, A431, Jurkat, rat spleen, and mouse liver lysates.

Western Blotting Analysis: A 1:500-1:2,000 dilution of this antibody detected PAX3 in A549, A431, Jurkat, rat spleen, and mouse liver lysates.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~53 kDa observed. Uncharacterized bands may be observed in some lysate(s).